Which of the following might lead to the generation of several incorrect PCR products?
A) An annealing temperature that is too low
B) An annealing temperature that is too high
C) Performing 30 cycles of PCR
D) Using the correct primers
E) Including an extension step

Question

Quizplus · Accepted Answer

An annealing temperature that is too low can lead to non-specific binding of primers, resulting in the generation of multiple incorrect PCR products.

Which of the Following Might Lead to the Generation of Several