In Addition to Noncoding Sequences, Most Eukaryotic Genomes Contain Not

In addition to noncoding sequences, most eukaryotic genomes contain not only functional genes that encode proteins, but also nonfunctional pseudogenes. Pseudogenes are copies of functional genes, but contain mutations that prevent function or could block either transcription or translation. Which of the following could indicate that a gene-like DNA sequence is a pseudogene sequence and not a functioning gene?

A) The sequence has no transcription-factor binding sites.
B) The sequence has no putative open reading frame.
C) The sequence is not transcribed.
D) The sequence has introns and spliceosome-binding sites.
E) The sequence folds back on itself to form a hairpin.