Calculate the WBC count in the following from the information provided:
-Using an undiluted specimen,100 cells are counted in the nine large squares on one side of the hemocytometer.

Question

Quizplus · Accepted Answer

To calculate the WBC count, we need to use the following formula:

Number of cells counted ÷ Volume of fluid counted ÷ Dilution factor = Number of cells/µL

Here, we have counted 100 cells in nine large squares. Each large square represents an area of 1 mm2 (0.1 x 0.1 mm). Therefore, the total area counted on one side of the hemocytometer is 9 mm2.

Assuming there was no dilution of the specimen (since it was an undiluted specimen), the volume of fluid counted is 0.1 mL (or 100 µL).

Plugging in the numbers:

100 cells ÷ 0.1 mL ÷ 1 = 1000 cells/µL

Therefore, the WBC count is 1000 cells/µL. The closest answer choice is B (111 cells/µL), which is an incorrect calculation.

Calculate the WBC Count in the Following from the Information