The process of generating monoclonal antibodies is labor-intensive and expensive.An alternative is to use polyclonal antibodies.A subpopulation of purified polyclonal antibodies that recognize a particular antigen can be isolated by chromatography.Which type of chromatography is used for this purpose?

Question

Quizplus · Accepted Answer

Affinity chromatography is the best choice for isolating a subpopulation of purified polyclonal antibodies that recognize a particular antigen. Affinity chromatography uses a matrix that is covalently linked to the antigen or a molecule that mimics the antigen, allowing for selective binding and purification of the desired antibodies. Ion-exchange and gel-filtration chromatography can also be used in the purification process, but they are not as selective as affinity chromatography.

The Process of Generating Monoclonal Antibodies Is Labor-Intensive and Expensive