Which of the following methods would be the most suitable to assess whether your protein exists as a monomer or in a complex?

Question

Quizplus · Accepted Answer

Gel-filtration chromatography separates proteins based on size, allowing for identification of whether a protein exists as a monomer or in a complex. Gel electrophoresis and western blot analysis can provide information on protein size, but may not clearly indicate whether a protein is present as a monomer or in a complex. Ion-exchange chromatography separates proteins based on charge, which may not be the best indicator of protein quaternary structure.

Which of the Following Methods Would Be the Most Suitable