Studies conducted with a lysozyme mutant that contains an Asp→Asn change at position 52 and a Glu→Gln change at position 35 exhibited almost a complete loss in enzymatic activity.What is the most likely explanation for the decrease in enzyme activity in the mutant?

Question

Quizplus · Accepted Answer

The Asp→Asn and Glu→Gln changes result in the loss of negative charges at the active site, which are crucial for substrate binding and catalysis, leading to a decrease in enzymatic activity.

Studies Conducted with a Lysozyme Mutant That Contains an Asp→Asn