You have produced a monoclonal antibody that binds to the protein actin.To be sure that the antibody does not cross-react with other proteins, you test your antibody in a western blot assay on whole-cell lysates that have been subjected to electrophoresis under nondenaturing conditions (shown in Figure 4-69A) and denaturing conditions (shown in Figure 4-69B).Does the antibody cross-react with other proteins? If so, does this explain the results in the two western blots? If not, how do you explain the difference observed?
Figure 4-69

Question

You have produced a monoclonal antibody that binds to the protein actin.To be sure that the antibody does not cross-react with other proteins, you test your antibody in a western blot assay on whole-cell lysates that have been subjected to electrophoresis under nondenaturing conditions (shown in Figure 4-69A) and denaturing conditions (shown in Figure 4-69B).Does the antibody cross-react with other proteins? If so, does this explain the results in the two western blots? If not, how do you explain the difference observed?

Figure 4-69

Quizplus · Accepted Answer

The Answer of You have produced a monoclonal antibody that binds to the protein actin.To be sure that the antibody does not cross-react with other proteins, you test your antibody in a western blot assay on whole-cell lysates that have been subjected to electrophoresis under nondenaturing conditions (shown in Figure 4-69A) and denaturing conditions (shown in Figure 4-69

You Have Produced a Monoclonal Antibody That Binds to the Protein