The mouse genome consists of 2,700 Mb. A genomic library is constructed using BAC vectors and overlapping fragments of the mouse genome. The average size of the mouse DNA inserts is 200 kb.
-A mouse genomic library is constructed so that the number of clones equals one genomic equivalent.Is it likely that this library contains the entire mouse genome?
A)No; by chance some parts of the genome will be represented more than once and others not at all.
B)Yes; that is a sufficient number clones for every part of the genome to be represented one time.
C)No; the restriction enzyme used to fragment the genome will delete some DNA on the ends of clones.

Question

Quizplus · Accepted Answer

A genomic library with a coverage of one genomic equivalent does not guarantee that every part of the genome will be represented due to the random nature of fragment selection and cloning. Some regions may be cloned multiple times, while others might be missed entirely.

The Mouse Genome Consists of 2,700 Mb