You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace.
-What is the sequence of the DNA synthesized in the sequencing reaction?
A)5' TTTGCTTTGTGAGCGGATAACAA 3'
B)3' TTTGCTTTGTGAGCGGATAACAA 5'
C)5' AAACGAAACACTCGCCTATTGTT 3'
D)3' AAACGAAACACTCGCCTATTGTT 5'

Question

You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace. 
-What is the sequence of the DNA synthesized in the sequencing reaction?
A)5' TTTGCTTTGTGAGCGGATAACAA 3' 
B)3' TTTGCTTTGTGAGCGGATAACAA 5' 
C)5' AAACGAAACACTCGCCTATTGTT 3' 
D)3' AAACGAAACACTCGCCTATTGTT 5'

Quizplus · Accepted Answer

The sequence of the DNA synthesized in the sequencing reaction is complementary to the template strand. The template strand is 3' TTTGCTTTGTGAGCGGATAACAA 5', so the sequence of the DNA synthesized in the sequencing reaction is 5' TTTGCTTTGTGAGCGGATAACAA 3'.

You Performed a Sanger Sequencing Reaction and Obtained the Following