You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace.
-Which is a step in hierarchical genome sequencing that is not found in shotgun genome sequencing?
A)fragment the genome
B)construct a genomic library
C)organize the clones based on their order in the genome
D)sequence the ends of millions of clones
E)assemble the sequences into contigs based on overlap

Question

You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace. 
-Which is a step in hierarchical genome sequencing that is not found in shotgun genome sequencing?
A)fragment the genome 
B)construct a genomic library 
C)organize the clones based on their order in the genome 
D)sequence the ends of millions of clones 
E)assemble the sequences into contigs based on overlap

Quizplus · Accepted Answer

Hierarchical genome sequencing involves organizing the clones based on their order in the genome, a step not found in shotgun sequencing. Shotgun sequencing randomly fragments the genome and sequences those fragments without prior organization.

You Performed a Sanger Sequencing Reaction and Obtained the Following