You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace.
-What is the sequence of the template DNA used for this sequencing reaction?
A)5' TTTGCTTTGTGAGCGGATAACAA 3'
B)3' TTTGCTTTGTGAGCGGATAACAA 5'
C)5' AAACGAAACACTCGCCTATTGTT 3'
D)3' AAACGAAACACTCGCCTATTGTT 5'

Question

You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace. 
-What is the sequence of the template DNA used for this sequencing reaction?
A)5' TTTGCTTTGTGAGCGGATAACAA 3' 
B)3' TTTGCTTTGTGAGCGGATAACAA 5' 
C)5' AAACGAAACACTCGCCTATTGTT 3' 
D)3' AAACGAAACACTCGCCTATTGTT 5'

Quizplus · Accepted Answer

The template DNA is complementary and antiparallel to the newly synthesized strand. The sequence provided is the newly synthesized strand, so the template must be the reverse complement of that sequence.

You Performed a Sanger Sequencing Reaction and Obtained the Following