Question 1

Recombinant vaccines have been developed using_________ .

Accepted Answer

The answer of Recombinant vaccines have been developed using_________ ....

Question 2

The host for a BAC is typically a mutant strain of Escherichia coli that is missing_________ and is defective in __________.

Accepted Answer

The answer of The host for a BAC is typically...

Question 3

It is usually desirable to design a vector that transcribes cloned genes at high levels at all times.

Accepted Answer

Constant high-level transcription of cloned genes can lead to metabolic burden, resource depletion, and potential toxicity in host cells, making it undesirable for many applications.

Question 4

One limitation to designing an antibody for detecting a particular protein is that the antibody must be_________ for the protein of interest.

Accepted Answer

The answer of One limitation to designing an antibody for...

Question 5

One important advantage of eukaryotic cells as hosts for cloning vectors is that they already possess the complex RNA and posttranslational processing systems required for the production of eukaryotic proteins.

Accepted Answer

Eukaryotic cells possess the necessary molecular machinery for post-translational modifications such as glycosylation, phosphorylation, and acetylation, which are required for the proper folding and function of eukaryotic proteins. This makes them advantageous hosts for cloning vectors that aim to produce and express eukaryotic proteins.

Question 6

Although a gene library may contain tens of thousands of clones, finding the clone of interest is the biggest challenge.

Accepted Answer

This is because the process of screening and isolating the clone of interest from a gene library can be complex and time-consuming. Various techniques such as hybridization, PCR, and sequencing are used to identify the specific clone carrying the gene of interest.

Question 7

A(n)_________ uses a DNA or RNA probe to detect the presence of a particular DNA sequence. A(n) _________uses a DNA or RNA probe that is used to detect the presence of a particular RNA sequence.

Accepted Answer

The answer of A(n)_________ uses a DNA or RNA probe...

Question 8

A major disadvantage of using Bacillus subtilis as a cloning host is that over several generations of subculturing,_________ .

Accepted Answer

The answer of A major disadvantage of using Bacillus subtilis...

Question 9

Artificial chromosomes are vectors capable of carrying_________ .

Accepted Answer

The answer of Artificial chromosomes are vectors capable of carrying_________...

Question 10

In principle, a type II restriction endonuclease with an 8- nucleotide recognition sequence should cut 1 in every 48 nucleotide positions.

Accepted Answer

A type II restriction endonuclease recognizes a specific palindromic sequence and cuts within or near the recognition sequence. If the recognition sequence is 8 nucleotides long, there are 4^8 = 65536 possible recognition sequences. Therefore, in principle, a type II restriction endonuclease with an 8-nucleotide recognition sequence should cut 1 in every 48 nucleotide positions.

Question 11

One problem with both BACs and YACs is that genetic regions of these chromosomes cannot be subcloned.

Accepted Answer

Both BACs (Bacterial Artificial Chromosomes) and YACs (Yeast Artificial Chromosomes) are used precisely because they allow for the subcloning of large fragments of DNA, enabling detailed genetic analysis and manipulation.

Question 12

If the gene in question is not expressed and no assay or antibody is available to detect the gene product, the most common procedure used for identification is a(n)_________ probe.

Accepted Answer

The answer of If the gene in question is not...

Question 13

One method of avoiding false positive results during hybridization reactions is to adjust the_________ of the hybridization, such that complementarity between the sequence of the gene of interest and the probe must be nearly exact.

Accepted Answer

The answer of One method of avoiding false positive results...

Question 14

Banding patterns generated from restriction analyses of either whole chromosomes or specific genes from a series of organisms can yield an indication of their genetic relationships.

Accepted Answer

Restriction analyses can reveal similarities and differences in DNA sequences between different organisms, allowing for inferences about their genetic relationships.

Question 15

Codon bias offers clues about reading frame functionality because most organisms show preferences among_________ .

Accepted Answer

The answer of Codon bias offers clues about reading frame...

Question 16

Lambda is a particularly useful cloning vector because _________and __________ .

Accepted Answer

The answer of Lambda is a particularly useful cloning vector...

Question 17

A cloning vector that can stably replicate in two unrelated host organisms is known as a(n)_________.

Accepted Answer

The answer of A cloning vector that can stably replicate...

Question 18

The process by which a fragment of DNA is placed into a vector where it can be replicated is called _________.

Accepted Answer

The answer of The process by which a fragment of...

Question 19

One basic technique of genetic engineering includes the ability to cut the DNA of interest into random fragments.

Accepted Answer

Genetic engineering techniques involve cutting DNA into specific fragments, not random fragments.

Question 20

Genetic engineering includes techniques that result in genetically modified organisms.

Accepted Answer

Genetic engineering involves modifying the genetic material of an organism, resulting in genetically modified organisms (GMOs).

Quiz 11: Genetic Engineering