A protein of interest can be specifically detected on western blots; this technique requires all of the following EXCEPT

Question

Quizplus · Accepted Answer

Labeled DNA is not required for western blot analysis. Instead, the basic requirements are primary antibodies specific to the protein of interest, enzyme-tagged secondary antibodies to bind to the primary antibodies, and an easily detectable product formed by the enzyme in the tagged secondary antibody. Additionally, antibodies can be used to detect the amount and size of specific proteins in cell extracts.

A Protein of Interest Can Be Specifically Detected on Western