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DNA Protection Assays Identify Specific Nucleotide-Protein Contacts

Question 39

Multiple Choice

DNA protection assays identify specific nucleotide-protein contacts. What is the role of deoxyribonuclease (DNase) I treatment in footprint analysis?


A) DNase I is used to break DNA previously bound to protein.
B) Protein-bound DNA is protected from DNase I and it can be isolated and characterized.
C) DNase I prepares protein-bound DNA for sequencing.
D) DNase I is used to cleave Taq polymerase.
E) DNase I synthesizes DNA from the upstream primer.

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