In the yeast two-hybrid analysis technique, in vivo protein-protein interactions are explored. The "bait" and "prey" proteins are translationally fused to the cleaved activation and DNA-binding domains of the GAL4 transcription factor. What is the target gene of GAL4?

Question

Quizplus · Accepted Answer

The target gene of GAL4 is the GAL1 gene, which encodes for the enzyme galactokinase. When GAL4 is active, it binds to the upstream activating sequence (UAS) of the GAL1 gene, leading to its transcription and subsequent translation of galactokinase protein. This is the basis of the yeast two-hybrid technique, where interaction between bait and prey proteins leads to activation of GAL4, which in turn leads to activation of the GAL1 gene and production of galactokinase enzyme.

In the Yeast Two-Hybrid Analysis Technique, in Vivo Protein-Protein Interactions