Hepatocytes Are Isolated from Biopsy Samples Acquired from Patients Getting

Hepatocytes are isolated from biopsy samples acquired from patients getting standard treatment at a local tertiary referral center as part of a research project studying enzyme activity in both normal and sick liver tissue. To remove membrane components and organelles, the cells are homogenized and centrifuged. Following several centrifugation rounds, the residual supernatant comprises solely cytosol and cytosolic proteins. Which of the following enzymes' activity is most likely to be detectable in the supernatant of healthy liver cells?

A) 3-Hydroxy-3-methylglutaryl-CoA lyase
B) Ornithine transcarbamylase
C) Pyruvate carboxylase
D) Succinate dehydrogenase
E) Transketolase