Which of the Following Conditions Is NOT Required for the Determination

Which of the following conditions is NOT required for the determination of the 'glycaemic index' (GI) of food product 'Q'?

A) At least 10 healthy people are fed a reference food (either glucose or white bread) and product Q on two separate occasions.
B) On each occasion, reference food and product Q are given in an equal amount (usually fixed portion of 50 g) of available carbohydrate.
C) After the ingestion of reference food and product Q, blood samples are collected every two hours for 24 hours, and blood glucose concentrations are measured.
D) For each person, a GI value for product Q is obtained by dividing the area under the blood glucose response curve (AUC) for product Q by the glucose AUC for the reference food.
E) The final GI value for product Q is the average GI value from all the participants.