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A Scientist Used a Mammalian Cell Extract to See Whether

Question 100

Multiple Choice

A scientist used a mammalian cell extract to see whether it contained any chromatin remodeling factors. She incubated the cell extract with and without ATP in the presence of chromatin containing promoters X, Y, and Z and tested whether RNA polymerase would bind to any of the promoters. The table below summarizes the results. Do the results suggest that a chromatin remodeling factor was present in the cell extract?  RNA polymerase binding  Promoter  + ATP -ATP X Yes  No  Y  Yes  Yes Z No  No \begin{array} { c } \quad\quad{ \text { RNA polymerase binding } } \\\begin{array} { | c | c | c | } \hline \text { Promoter } & \text { + ATP }\quad\quad\quad\quad & \text {-ATP } \quad\quad\quad\\\hline \mathrm { X } & \text { Yes } & \text { No } \\\hline \text { Y } & \text { Yes } & \text { Yes } \\\hline \mathrm { Z } & \text { No } & \text { No } \\\hline\end{array}\end{array}


A) Yes, because Promoter Y binds RNA polymerase independent of the presence of ATP.
B) Yes, because Promoter X binds RNA polymerase only when ATP is present.
C) No, because Promoter Z cannot bind RNA polymerase even when ATP is absent.
D) No, because there is not enough data to tell whether a chromatin remodeling factor may be present.

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