A protein mixture is commonly applied to an HIC column under high ___ conditions, and the protein of interest is eluted with buffer of decreasing ____.

Question

Quizplus · Accepted Answer

Hydrophobic interaction chromatography (HIC) typically involves applying the protein mixture to the column under high salt conditions to promote binding of proteins based on their hydrophobicity. The protein of interest is then eluted by decreasing the ionic strength of the buffer, which weakens the hydrophobic interactions and allows the proteins to elute.

A Protein Mixture Is Commonly Applied to an HIC Column