Which is NOT true of a cellular protein with an acidic isoelectric point?
A) Its pI (isoelectric point) is likely at pH 1 or 2
B) It contains more anionic amino acid residues than cationic amino acid residues
C) At neutral pH, its carboxyl groups would be deprotonated
D) It would bind to a positively charged ion-exchange resin
E) It would be best to use an anion exchange resin to separate this cellular protein
Correct Answer:
Verified
Q43: A C-8 column would preferentially bind
A) strongly
Q44: A protein mixture is commonly applied to
Q45: Gradients allow a gradual change in buffer
Q46: How does reverse-phase chromatography differ from HIC?
A)
Q47: Ion exchange chromatography
I. separates proteins from a
Q49: Carboxymethyl (CM) ion-exchange resin
A) bears a positive
Q50: A researcher adds a "tag" of several
Q51: Metal affinity chromatography (MAC) commonly uses divalent
Q52: Like ion exchange chromatography, MAC
A) eluent buffer
Q53: Biotin's cellular function includes
A) fatty acid biosynthesis
B)
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