How does reverse-phase chromatography differ from HIC?
A) In HIC, eluting buffers can be gentler
B) In HIC, the hydrophobic interactions between proteins and resin are weaker
C) In HIC, the hydrophobic interactions between proteins and resin are stronger
D) In reverse-phase, low MW proteins elute from the column earliest
E) Both A and B
Correct Answer:
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Q41: Resins used in affinity chromatography commonly form
Q42: Sepharose resin modified with a butyl or
Q43: A C-8 column would preferentially bind
A) strongly
Q44: A protein mixture is commonly applied to
Q45: Gradients allow a gradual change in buffer
Q47: Ion exchange chromatography
I. separates proteins from a
Q48: Which is NOT true of a cellular
Q49: Carboxymethyl (CM) ion-exchange resin
A) bears a positive
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