Ion exchange chromatography
I. separates proteins from a mixture based on each protein's isoelectric point
II. depends on the pH at which a protein is electronically neutral
III. depends on the pH at which the positively charged residues of a protein
Equal the negatively charged residues
IV. uses coulombic effects to separate proteins

Question

Quizplus · Accepted Answer

Ion exchange chromatography separates proteins based on their charge, which is influenced by the protein's isoelectric point (I), the pH at which the protein is electrically neutral (II), and the balance of positively and negatively charged residues (III). Coulombic (electrostatic) effects are fundamental to this separation process (IV).

Ion Exchange Chromatography I. Separates Proteins from a Mixture Based on Each Protein's