Like ion exchange chromatography, MAC
A) eluent buffer is an imidazole salt
B) eluent buffer contains high concentrations of histidine
C) eluent buffer competes with bound protein for resin binding sites
D) depends on increased pH to elute the bound protein
E) never uses a gradient eluent buffer
Correct Answer:
Verified
Q47: Ion exchange chromatography
I. separates proteins from a
Q48: Which is NOT true of a cellular
Q49: Carboxymethyl (CM) ion-exchange resin
A) bears a positive
Q50: A researcher adds a "tag" of several
Q51: Metal affinity chromatography (MAC) commonly uses divalent
Q53: Biotin's cellular function includes
A) fatty acid biosynthesis
B)
Q54: Which is NOT true of biotin-avidin interaction?
A)
Q55: A researcher biotinylates a protein; this protein
Q56: A biotinylated protein likely will not be
Q57: The most common immunoaffinity column packing is
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