A researcher adds a "tag" of several histidine residues to an engineered protein in order to purify the protein using
A) size-exclusion chromatography
B) MAC
C) an immunoaffinity column
D) a cation exchange column
E) HIC
Correct Answer:
Verified
Q45: Gradients allow a gradual change in buffer
Q46: How does reverse-phase chromatography differ from HIC?
A)
Q47: Ion exchange chromatography
I. separates proteins from a
Q48: Which is NOT true of a cellular
Q49: Carboxymethyl (CM) ion-exchange resin
A) bears a positive
Q51: Metal affinity chromatography (MAC) commonly uses divalent
Q52: Like ion exchange chromatography, MAC
A) eluent buffer
Q53: Biotin's cellular function includes
A) fatty acid biosynthesis
B)
Q54: Which is NOT true of biotin-avidin interaction?
A)
Q55: A researcher biotinylates a protein; this protein
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