You are examining the regulatory control of glycolysis by addition of a novel compound to a hepatocyte cell culture system. You discover that addition of the compound results in an increased rate of ATP production from glucose. Further studies demonstrate that the changes observed in your culture system with addition of the compound can be mimicked by a number of substances that activate the rate-limiting enzyme of glycolysis. Your compound is most likely altering the rate of glycolysis in a manner similar to all of which of the following?

Question

Quizplus · Accepted Answer

AMP and fructose 2,6-bisphosphate are known activators of phosphofructokinase-1 (PFK-1), the rate-limiting enzyme of glycolysis, leading to increased glycolytic flux and ATP production. Inorganic phosphate also supports glycolysis by participating in substrate-level phosphorylation. Allosteric activation of PFK1 would be expected to occur under conditions of low-energy charge. Thus, as the level of ATP drops, there will be a concomitant rise in both AMP and inorganic phosphate levels. Both serve to allosterically activate PFK1. PFK1 is also allosterically activated by fructose-2,6-bisphosphate whose levels also rise in response to changing energy levels.

You Are Examining the Regulatory Control of Glycolysis by Addition