You are examining the regulatory control of glycolysis by addition of a novel compound to a hepatocyte cell culture system. You discover that addition of the compound results in an increased rate of ATP production from glucose while simultaneously reducing glucose output into the culture medium. Your experimental compound is most likely mimicking which of the following?

Question

Quizplus · Accepted Answer

Fructose 2,6-bisphosphate is a potent activator of phosphofructokinase-1 (PFK-1), a key regulatory enzyme in glycolysis, which increases the rate of glycolysis and ATP production. It also inhibits gluconeogenesis, reducing glucose output.  Fructose-2,6-bisphosphate (F2,6BP) is the most potent allosteric activator of PFK1, resulting in activation of glycolysis. Simultaneously, F2,6BP allosterically inhibits the gluconeogenic enzyme F-1,6-Bpase

You Are Examining the Regulatory Control of Glycolysis by Addition