The strength and type of charge on the hemoglobin molecule will enable the separation of hemoglobin variants by electrophoresis.What other significant factor controls this separation?

Question

Quizplus · Accepted Answer

The pH of the medium is a significant factor that controls the separation of hemoglobin variants by electrophoresis. The charge on the hemoglobin molecule is pH-dependent, and a change in pH can alter the strength and type of charge on the molecule, thereby affecting its electrophoretic mobility. Therefore, the pH of the medium is an important factor that needs to be controlled to ensure optimal separation of hemoglobin variants. The other factors listed (solubility, heat precipitation, and elevation of concentration) are not directly related to the electrophoretic separation of hemoglobin variants.

The Strength and Type of Charge on the Hemoglobin Molecule