Real-time PCR can be used for which of the following?
A)Conduct a PCR reaction without the use of primers
B)Generate cDNA
C)Quantify expression of a specific gene in a cell
D)Nonspecific DNA amplification

Question

Quizplus · Accepted Answer

Real-time PCR, also known as quantitative PCR, can be used to quantify the expression of a specific gene in a cell. This is achieved by using fluorescent dyes or probes that bind to the amplified product, allowing for real-time monitoring of the reaction. Real-time PCR cannot be used for conducting a PCR reaction without primers, generating cDNA, or nonspecific DNA amplification.

Real-Time PCR Can Be Used for Which of the Following