You are working to characterize a novel protein in mice.Characterization of the mRNA expression pattern revealed that the gene is highly expressed in brain,muscle,liver,and pancreas.To determine the size of the corresponding protein,you make protein extracts from each of the tissues.Using an antibody that recognizes the C-terminal portion of the protein,you perform an analysis that allows you to visualize the extracted protein.Interestingly,you can detect the protein in brain,muscle,and liver,but not the pancreas.Assuming your extracts and reagents are good,and there is no human error,what is the most likely explanation for this result?
A)Transcription levels in pancreas are too low.
B)Translation levels in pancreas are too low.
C)Alternative splicing in the pancreas leads to protein degradation.
D)Alternative splicing in the pancreas yields a protein missing the portion of the protein to which the antibody was raiseD.

Question

Quizplus · Accepted Answer

The fact that the protein was not detected in the pancreas despite high mRNA expression suggests that alternative splicing may have occurred in this tissue. The alternative splicing could have resulted in a protein that is missing the portion recognized by the antibody used in the analysis.

You Are Working to Characterize a Novel Protein in Mice