Calibration of a spectrophotmeter using a series of green dye containing solutions provides a set of data which follows the Beer-Lambert Law,with a trendline of y = 1.070 $\times$10³x (y-axis = absorbance,x-axis = molar concentration).What is the molar concentration of a solution with an absorbance of 0.3131?

Question

Quizplus · Accepted Answer

The Beer-Lambert Law is given by A = εlc, where A is absorbance, ε is the molar absorptivity, l is the path length, and c is the concentration. From the trendline equation y = 1.070 × 10^3 x, ε = 1.070 × 10^3. Solving for c when A = 0.3131 gives c = 0.3131 / (1.070 × 10^3) = 0.2926 M.

Calibration of a Spectrophotmeter Using a Series of Green Dye ×\times×

Calibration of a Spectrophotmeter Using a Series of Green Dye $\times$