Question 1

List the order of enzymes,enzyme complexes,and membrane soluble electron carriers that an electron is passed through on its way from NADH to O₂ in the electron transport system.

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Question 2

Describe the process by which proton flow through the c subunits forces the rotation of the ATP synthase spindle in the mitochondrial membrane.Please include the roles of aspartic acid residues and the half-channels in your description.

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Question 3

List the conformation types and the changes to the substrates in a single subunit on the ATP synthase catalytic head piece during one 360^o rotation of the rotor.

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Question 4

Add to the malate-aspartate shuttle scheme below the missing reactant and products a,b,c,and
d.

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Question 5

Explain why many mitochondrial diseases are inherited from the mother only.

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Question 6

Explain how ATP synthase can be used as primary active transporter of H⁺ and pump protons.

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Question 7

Why are 2,4-dintrophenol and thermogenin referred to as uncouplers?

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Question 8

Which directions across the mitochondria must H⁺,ATP,ADP,and P_i travel during active mitochondrial ATP synthesis?

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Question 9

For each molecule below,list the number of ATP obtained assuming that the electron transport chain and oxidative phosphorylation are active. NADH (from the TCA cycle) FADH₂ (from the TCA cycle) NADH (from the cytoplasm,malate aspartate shuttle) NAD⁺ (from the mitochondria)

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Question 10

The structure of 2,4-dinitrophenol is shown below.Explain how it functions to uncouple a proton gradient across the mitochondrial membrane.

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Question 11

List the common enzyme names of the major enzyme complexes in the oxidative phosphorylation pathway,which are required to couple NADH oxidation with ATP synthesis.

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Question 12

Explain the origin of the brown color of brown adipose tissue.

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Question 13

The free energy released from the electron transport system reaction NADH + ¹/₂ O₂ + H⁺ H₂O + NAD⁺ is '= -220 kJ/mol.How many moles of ATP could theoretically be made assuming 100% efficiency in energy coupling through the chemiosmotic theory,and that ADP+P_i ATP '= 30.5 kJ/mol?

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Question 14

Label the locations of , , ,and c,and the F₀ and F₁ subunits on the following ATP synthase enzyme.

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Question 15

On the figure below label the clearly identify the positions of the three functional units of ATP synthase: the rotor,the catalytic headpiece,and the stator.

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Question 16

List the major enzymes and enzyme complexes in the oxidative phosphorylation pathway,which are required to couple FADH₂ oxidation with ATP synthesis.

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Question 17

Draw a diagram of the mitochondrion and indicate which side of the organelle has a net positive charge and which side has a net negative charge as a result of proton pumping.

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Question 18

Use the structure of the oxidized coenzyme Q (ubiquinone)shown below and draw the two-electron reduced form.

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Quiz 11: Oxidative Phosphorylation

List the order of enzymes,enzyme complexes,and membrane soluble electron carriers that an electron is passed through on its way from NADH to O2 in the electron transport system.

Describe the process by which proton flow through the c subunits forces the rotation of the ATP synthase spindle in the mitochondrial membrane.Please include the roles of aspartic acid residues and the half-channels in your description.

List the conformation types and the changes to the substrates in a single subunit on the ATP synthase catalytic head piece during one 360o rotation of the rotor.

Add to the malate-aspartate shuttle scheme below the missing reactant and products a,b,c,and d.