Which of the following statements is NOT characteristic of kcat/Km?
A) It corresponds to a second-order rate constant.
B) It provides an excellent parameter for comparison of the catalytic efficiency of enzymes.
C) It reflects the property of the enzyme when substrate concentration is at saturation.
D) The upper limit for the kcat/Km value is fixed by the diffusion-controlled limit for reactions, which is 109 M−1 s−1.
E) It is also referred to as the turnover number.
Correct Answer:
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