If the pBR322 were treated with PstI in order to insert a gene into the plasmid, which of the following would be correct regarding cells that were transformed with the resulting vector?

Question

Quizplus · Accepted Answer

PstI cuts the pBR322 plasmid between the ampicillin and tetracycline resistance genes, creating two fragments with each of the resistance genes on a separate fragment. After insertion of the gene of interest, cells transform with the plasmid will only be resistant to tetracycline since the ampicillin resistance gene is on the other fragment that was not inserted. Therefore, cells that contain the desired vector would be resistant to tetracycline but sensitive to ampicillin.

If the PBR322 Were Treated with PstI in Order to Insert