The Correct Sequence for Colony Hybridization Experiments Is

The correct sequence for colony hybridization experiments is:
A.A replica of the bacterial colonies is obtained on an absorbent disC.
B.Autoradiography of the disc reveals probe complementary DNA.
C.Host bacteria with plasmid are plated and allowed to grow overnight.
D.The disc is treated with alkali.
E.The disc is reacted with labeled probe.

A) A, C, E, B, D
B) C, A, E, D, B
C) C, E, A, B, D
D) C, A, E, B, D
E) C, A, D, E, B