The correct sequence of procedures in the Southern blotting (hybridization) technique is:
A.hybridization with radioactive probE.
B.agarose gel electrophoresis and visualize bands.
C.transfer (blot) to nitrocellulose filter.
D.digest DNA with restriction nucleases.
E.expose filter to X-ray film, develop and observe.

Question

Quizplus · Accepted Answer

Southern blotting involves digesting DNA with restriction enzymes, separating the fragments by agarose gel electrophoresis, transferring them to a nitrocellulose filter, hybridizing with a radioactive probe, and then detecting the probe by exposing the filter to X-ray film.

The Correct Sequence of Procedures in the Southern Blotting (Hybridization)