The advantages of using the pET plasmid system include ____.
A) genes to be expressed are under the control of a T7 promoter
B) the T7 polymerase is slower than a typical E coli polymerase
C) the expressed protein never accounts for more than 5% of the total cell protein
D) the T7 polymerase is under the control of the lac operon and is thus only active when induced by an agent such as IPTG
E) both a and d are correct
Correct Answer:
Verified
Q1: One of the problems with plasmids like
Q2: Reporter genes such as those for green
Q3: The correct sequence for colony hybridization experiments
Q4: A genomic DNA library is:
A) a collection
Q5: If the pBR322 were treated with PstI
Q7: The correct sequence of procedures in the
Q8: All are characteristic of plasmids EXCEPT:
A) naturally
Q9: Shuttle vectors have the property that they:
A)
Q10: All are features of a plasmid useful
Q11: All are true for cDNA libraries EXCEPT:
A)
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