Recombination signal sequences are conserved heptamer and nonamer sequences that flank the V, J, and D gene segments which undergo recombination to generate the final V region coding exon. Some of these have 12-nucleotide spacers between the heptamer and nonamer, and others have 23-nucleotide spacers. The reason recombination signal sequences come in these two forms is:
A) To ensure the correct assembly of gene segments so that a VH recombines to a DH and not to another VH, for instance
B) To ensure that the heptamer and nonamer are found on the same face of the DNA double helix
C) To ensure that , , and heavy chains recombine within a locus and not between loci
D) To ensure that , , and heavy chain gene segments do not undergo recombination with non-immunoglobulin genes
E) To ensure that the RAG recombinase cuts the DNA between the last nucleotide of the heptamer and the coding sequence
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