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Recombination Signal Sequences Are Conserved Heptamer and Nonamer Sequences That κ\kappa

Question 16

Multiple Choice

Recombination signal sequences are conserved heptamer and nonamer sequences that flank the V, J, and D gene segments which undergo recombination to generate the final V region coding exon. Some of these have 12-nucleotide spacers between the heptamer and nonamer, and others have 23-nucleotide spacers. The reason recombination signal sequences come in these two forms is:


A) To ensure the correct assembly of gene segments so that a VH recombines to a DH and not to another VH, for instance
B) To ensure that the heptamer and nonamer are found on the same face of the DNA double helix
C) To ensure that κ\kappa , λ\lambda , and heavy chains recombine within a locus and not between loci
D) To ensure that κ\kappa , λ\lambda , and heavy chain gene segments do not undergo recombination with non-immunoglobulin genes
E) To ensure that the RAG recombinase cuts the DNA between the last nucleotide of the heptamer and the coding sequence

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