Question 1

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: The type of catalysis in which two substrates are brought into close proximity.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Approximation and orientation catalysis involves bringing two substrates close together to facilitate a reaction, often by orienting them in a way that makes the reaction more likely to occur.

Question 2

The mechanism of chymotrypsin involves the formation of an unstable ________________-shaped intermediate that is stabilized by the oxyanion hole.

Accepted Answer

tetrahedral

Question 3

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: A molecule that is also known as a substrate analog.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

An affinity label is a molecule that is designed to bind to an enzyme in a way that is similar to the enzyme's natural substrate. This binding causes irreversible inhibition of the enzyme, making an affinity label extremely effective at blocking enzyme activity.

Question 4

A ________________ inhibitor binds irreversibly to the active site of an enzyme.

Accepted Answer

The answer of A ________________ inhibitor binds irreversibly to the...

Question 5

The ________________ stabilizes the tetrahedral intermediate of the hydrolysis of a peptide bond by chymotrypsin.

Accepted Answer

The answer of The ________________ stabilizes the tetrahedral intermediate of...

Question 6

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: The inhibitor which binds only to the ES complex and lowers the Vmax and KM.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Uncompetitive inhibitors bind exclusively to the enzyme-substrate (ES) complex, leading to a decrease in both Vmax and KM.

Question 7

In conducting an experiment with a new drug, you find that regardless of the concentration of substrate, the drug is able to inhibit the enzyme activity. You are likely to not have a(n) ________________ type of inhibitor.

Accepted Answer

The answer of In conducting an experiment with a new...

Question 8

The straight-line kinetic plot of 1/V0 versus 1/S is called a ________________.

Accepted Answer

The answer of The straight-line kinetic plot of 1/V0 versus...

Question 9

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: An enzyme that is part of a pigment formation pathway and has a low optimum temperature.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Tyrosinase is the only enzyme listed that is part of a pigment formation pathway and has a low optimum temperature.

Question 10

An enzyme catalyst mechanism that uses a metal cation to stabilize a negative charge in the active site is ________________.

Accepted Answer

The answer of An enzyme catalyst mechanism that uses a...

Question 11

A ________________ catalytic mechanism that forces two substrates into an appropriate three-dimensional arrangement for the reaction to occur.

Accepted Answer

The answer of A ________________ catalytic mechanism that forces two...

Question 12

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: A type of enzyme inhibitor where KM is unaltered.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Noncompetitive inhibitors bind to an enzyme at a site other than the active site, which can alter the enzyme's activity without changing the affinity of the enzyme for its substrate (KM remains unchanged).

Question 13

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: The enzyme inhibition that can be overcome by increasing the concentration of substrate.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Competitive inhibition can be overcome by increasing the concentration of substrate.

Question 14

An uncompetitive inhibitor will have two ________________ lines on a double-reciprocal plot.

Accepted Answer

The answer of An uncompetitive inhibitor will have two ________________...

Question 15

The antibiotic penicillin is an example of a(n) ________________ inhibitor.

Accepted Answer

The answer of The antibiotic penicillin is an example of...

Question 16

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: The type of reaction catalyzed by proteases.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Proteases catalyze hydrolysis reactions, breaking down peptide bonds by adding a water molecule.

Question 17

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: A type of catalysis where the proton donor is not water.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

The answer of Use the following to answer questions
Choose the...

Question 18

A(n) ________________ inhibitor has a structure similar to the substrate and reversibly binds to the active site of the enzyme.

Accepted Answer

The answer of A(n) ________________ inhibitor has a structure similar...

Question 19

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: A protease enzyme with a low pH optimum.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Pepsin is a protease enzyme with a low pH optimum, as it is active in the acidic environment of the stomach. None of the other choices are specifically associated with a low pH optimum.

Question 20

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-____________: An enzyme that temporarily undergoes covalent catalysis as part of its mechanism.
A)hydrolysis
B)affinity label
C)tyrosinase
D)chymotrypsin
E)pepsin
F)noncompetitive
G)uncompetitive
H)heterotropic
I)approximation and orientation
J)acid-base
K)competitive

Accepted Answer

Chymotrypsin is an enzyme that temporarily undergoes covalent catalysis as part of its mechanism.

Quiz 8: Mechanisms and Inhibitors

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: The type of catalysis in which two substrates are brought into close proximity.

The mechanism of chymotrypsin involves the formation of an unstable ________________-shaped intermediate that is stabilized by the oxyanion hole.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: A molecule that is also known as a substrate analog.

A ________________ inhibitor binds irreversibly to the active site of an enzyme.

The ________________ stabilizes the tetrahedral intermediate of the hydrolysis of a peptide bond by chymotrypsin.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: The inhibitor which binds only to the ES complex and lowers the Vmax and KM.

In conducting an experiment with a new drug, you find that regardless of the concentration of substrate, the drug is able to inhibit the enzyme activity. You are likely to not have a(n) ________________ type of inhibitor.

The straight-line kinetic plot of 1/V0 versus 1/S is called a ________________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: An enzyme that is part of a pigment formation pathway and has a low optimum temperature.

An enzyme catalyst mechanism that uses a metal cation to stabilize a negative charge in the active site is ________________.

A ________________ catalytic mechanism that forces two substrates into an appropriate three-dimensional arrangement for the reaction to occur.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: A type of enzyme inhibitor where KM is unaltered.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: The enzyme inhibition that can be overcome by increasing the concentration of substrate.

An uncompetitive inhibitor will have two ________________ lines on a double-reciprocal plot.

The antibiotic penicillin is an example of a(n) ________________ inhibitor.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: The type of reaction catalyzed by proteases.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: A type of catalysis where the proton donor is not water.

A(n) ________________ inhibitor has a structure similar to the substrate and reversibly binds to the active site of the enzyme.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: A protease enzyme with a low pH optimum.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: An enzyme that temporarily undergoes covalent catalysis as part of its mechanism.